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Saturday, June 26, 2010

Transcription: Transcribe the following Enzyme-Related Acronyms



I.                   TRANSCRIPTION: ( 1 PT. EACH)
1.      NAD
2.      NADH
3.      LAP
4.      ALP
5.      ACP
6.      CK
7.      LD
8.      THC
9.      HPLC
10.  TDM


Monday, June 21, 2010

ANSWERS TO ENZYMOLOGY EXAM/REVIEW QUESTIONS

ANSWERS TO ENZYMOLOGY EXAM/REVIEW QUESTIONS


WRITE A IF TRUE AND C IF FALSE

89. “Foam test” is a test in amniotic fluid for fetal lung maturity. C – Foam test is for bilirubin
90. Wilson’s is a disease in copper metabolism. A
91. ALP is the enzyme class which uses NAD as a coenzyme. C-  enzyme involved is LDH
92. Transferases are enzymes which catalyzes the transfer of an amino group from one molecule to another. - A
93. Severe alteration of pH may cause denaturation. A
94. Higher than optimum temperature will cause denaturation. A
95. When an enzyme is measured at several intervals it is called the multipoint method. A
96. LD1 and LD2 are unstable at refrigerated temperatures. A
97. CK should be protected from light because it is photosensitive. A
98. CHS levels are usually depressed in liver diseases. A
99. The thyroid gland produces Calcitonin. A
100. the anterior pituitary produces Parathormone. C – parathyroid gland produces parathormone or PTH


Wednesday, June 16, 2010

Clinical Chemistry Exam/Review Questions - Enzymology Exams - ANSWERS 70 to 90.

ANSWERS TO QUESTIONS

Clinical Chemistry Exam/Review Questions - Enzymology Exams -
 
IDENTIFICATION: (Continuation)

70. In pesticide poisoning the enzyme affected is CHOLINESTERASE
71. Normal value for the answer to number 70. 6,000 TO 12,000 IU
72. Most common method for glucose determination is ORTHOTOLUIDINE METHOD
73. What is the concentration of albumin if globulin is 71 % of the TP, if TP=6.7?  4.69 mg/dL
74. The end product formed by the reaction of aspartate and oxoglutarate is oxaloacetate + L-glutamate
75. Enzymes are categorized into 6 classifications based on this.REACTION CATALYZED
76. When an enzyme requires an inorganic substance such as zinc for activity, this substrate is termed as ACTIVATOR
77. When substrate is in excess, this acts as the rate limiting factor.ENZYME CONCENTRATION
78. When the enzyme is in excess, this acts as the rate limiting factor.SUBSTRATE CONCENTRATION
79. These hormones have alpha chains that are biochemically identical but beta chains that are biochenically unique, FSH,TSH, HCG
80. This is the concentration of a substrate at half of its maximum velocity. MICHAELIS CONSTANT (kM)
81. What three enzymes are elevated in myocardial infarction? CPK,AST,LDH
82. Cretinism is caused by what hormone deficiency? GROWTH HORMONE
83. This anticoagulant is best for glucose determination because it prevents glycolysis. SODIUM FLUORIDE
84. CK has how many isoenzymes? 3
85. What CK isoenzyme is increased in brain injury? CKBB OR CK1
86. What is the principle of dry slide technology? REFLECTANCE SPECTROMETRY
87. What does the reagent blank corrects? ERRORS CAUSED BY THE COLOR OF REAGENT
88. Give the formula for the computation of Cu using the ratio to standard formula. ( 2 pts.) Cu= Au X Cs/ As


Saturday, June 12, 2010

Clinical Chemistry Exam/Review Questions - Enzymology Exams

Clinical Chemistry Exam/Review Questions - Enzymology Exams

IDENTIFICATION: (Continuation)

70. In pesticide poisoning the enzyme affected is ____________
71. Normal value for the answer to number 70.
72. Most common method for glucose determination is ____
73. What is the concentration of albumin if globulin is 71 % of the TP, if TP = 6.7?
74. The end product formed by the reaction of aspartate and oxoglutarate is ___
75. Enzymes are categorized into 6 classifications based on this.
76. When an enzyme requires an inorganic substance such as zinc for activity, this substrate is termed as_______
77. When substrate is in excess, this acts as the rate limiting factor.
78. When the enzyme is in excess, this acts as the rate limiting factor.
79. These hormones have alpha chains that are biochemically identical but beta chains that are biochenically unique,
80. This is the concentration of a substrate at half of its maximum velocity.
81. What three enzymes are elevated in myocardial infarction?
82. Cretinism is caused by what hormone deficiency?
83. This anticoagulant is best for glucose determination because it prevents glycolysis.
84. CK has how many isoenzymes?
85. What CK isoenzyme is increased in brain injury?
86. What is the principle of dry slide technology?
87. What does the reagent blank corrects?
88. Give the formula for the computation of Cu using the ratio to standard formula. ( 2 pts.)


WRITE A IF TRUE AND C IF FALSE

89. “Foam test” is a test in amniotic fluid for fetal lung maturity.
90. Wilson’s is a disease in copper metabolism.
91. ALP is the enzyme class which uses NAD as a coenzyme.
92. Transferases are enzymes which catalyzes the transfer of an amino group from one molecule to another.
93. Severe alteration of pH may cause denaturation.
94. Higher than optimum temperature will cause denaturation.
95. When an enzyme is measured at several intervals it is called the multipoint method.
96. LD1 and LD2 are unstable at refrigerated temperatures.
97. CK should be protected from light because it is photosensitive.
98. CHS levels are usually depressed in liver diseases.
99. The thyroid gland produces Calcitonin.
100. the anterior pituitary produces Parathormone.